U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX13249490: WGS Nanopore for Serratia marcescens: Felis catus urine
1 OXFORD_NANOPORE (MinION) run: 65,720 spots, 162.4M bases, 141.1Mb downloads

Design: Genomic DNA purified with Promega Wizard HMW Genomic DNA Extraction Kit. Libraries prepared with rapid barcoding kit SQK-RBK004 (Oxford Nanopore) and loaded on a MinION MK-Ib device fitted with flowcell R9.4. Live rapid basecalling performed with MinKnow 20.13.3 using Guppy version 4.2.2 (Oxford Nanopore), followed by filtering of reads with quality > 7, demultiplexing and barcode removal with guppy_barcoder (Oxford Nanopore). Reads > 1 kb processed with Filtlong (https://github.com/rrwick/Filtlong) using corresponding Illumina datasets as refernce sequences.
Submitted by: The University of Melbourne
Study: Nosocomial infections in a veterinary hospital
show Abstracthide Abstract
We examined a collection of Serratia spp. isolated from diverse patients at a large veterinary teaching hospital in Melbourne, Australia. We assessed their taxonomic identification, genomic diversity, and presence of antimicrobial resistance genes. We focused on a group of quasi-clonal strains isolated from complicated surgical procedures, which were found to be chlorhexidine tolerant and carried a IncHI2 plasmid containing multiple drug resistances.
Sample: Microbe sample from Serratia marcescens
SAMN23485796 • SRS11172836 • All experiments • All runs
Library:
Name: CM2012_028_n
Instrument: MinION
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: SINGLE
Runs: 1 run, 65,720 spots, 162.4M bases, 141.1Mb
Run# of Spots# of BasesSizePublished
SRR1706058365,720162.4M141.1Mb2022-03-01

ID:
18126739

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...